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Abstract:
Microalgae hold promise as sustainable and renewable resources for phenolic compound removal. Industrial mill wastewater often contained phenol. In this work, we isolated and identified a freshwater diatom through 18S DNA analysis, Navicula permitis. Then, we assessed its ability to grow and effectively eliminate phenolic compounds at concentrations ranging from 50 to 250 mg/L to address incomplete treatment issues. Chlorophyll fluorescence was measured under stress, and phenol degradation was examined through phenol hydroxylase and catechol dioxygenase activity in N. permitis. Our findings revealed that N. permitis could remove phenol concentrations up to 145.9 mg/L, with optimal conditions at 50.08 mg/L phenol, 106 cells/mL N. permitis, and 11.38 days of treatment. A zero-order kinetic model described the elimination of phenol. The metabolism mechanism supposed to biodegrade the phenolic compound in this experiment is that N. permitis may employ PHase. The metabolism of phenol included the ortho-pathway. N. permitis generated biomass while degrading phenol, making it a good choice for ecological remediation.
Keywords: phenolic compounds, wastewater, Navicula permitis, enzymes, biodegradation.
